Conformation of poly(dG-dC) . poly(dG-dC) modified by the carcinogens N-acetoxy-N-acetyl-2-aminofluorene and N-hydroxy-N-2-aminofluorene.
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منابع مشابه
Conformation of poly(dG-dC) . poly(dG-dC) modified by the carcinogens N-acetoxy-N-acetyl-2-aminofluorene and N-hydroxy-N-2-aminofluorene.
Poly(dG-dC) . poly(dG-dC) was modified by reaction with N-acetoxy-N-acetyl-2-aminofluorene (N-AcO-AAF). Two samples with 6.6% and 8.5% modified bases were prepared. The modified bases are randomly distributed along the polymer chain, as deduced from competition experiments between antibodies against N-2-(guanosin-8-yl)-acetylaminofluorene, modified poly(dG-dC) . poly(dG-dC), and modified DNAs. ...
متن کاملN-2-Aminofluorene Adducts
The Escherichia coli UvrABC endonuclease is known to be involved in the repair of DNA damage induced by UV irradiation (6, 11, 23, 25) or a large number of chemical agents (1, 10, 12, 19, 24). This enzyme, termed excinuclease (12, 19), is constituted by three subunits. UvrA binds weakly to DNA in an ATP-dependent manner, UvrB binds to UvrA and DNA to form a stable complex which scans the DNA (1...
متن کاملCharacterization of mutations induced by 2-(N-acetoxy-N-acetyl)aminofluorene in the dihydrofolate reductase gene of cultured hamster cells.
To determine the types of alterations in gene structure that are induced by the carcinogen 2-(N-acetoxy-N-acetyl)aminofluorene, we used this compound to generate mutations at the dihydrofolate reductase (DHFR) locus (DHFR) in Chinese hamster ovary cells. Twenty-nine independent enzyme-deficient mutants were isolated. A profile of the 26-kilobase (kb)-long gene was obtained by Southern blot anal...
متن کاملConformational changes induced in DNA by in vitro reaction with N-hydroxy-N-2-aminofluorene.
The conformation of DNA modified in vitro by the covalent binding of N-OH-AF was investigated by ultraviolet absorbance, circular dichroism and by radioimmunoassay using specific antibodies against Guo-AAF and nDNA-AAF. The results obtained by both physico-chemical and immunological methods are in agreement with a model involving destabilized regions in the double helical DNA around the carcino...
متن کاملInteraction of DNA methyltransferase with aminofluorene and N-acetylaminofluorene modified poly(dC-dG).
Poly(dC-dG) was reacted in vitro to yield templates containing similar amounts of aminofluorene (AF) or acetylaminofluorene (AAF) adducts. These modified poly(dC-dG) templates were tested in an in vitro DNA methylation system utilizing 1500-fold purified rat liver methyltransferase (DMase) to compare and quantitate the effects of these adducts on the kinetics of methylation and the interaction ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1980
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.77.8.4597